ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Plant regeneration from protoplasts of Capsicum annuum L cv. California Wonder

Prakash, AH and Rao, K Sankara and Kumar, M Udaya (1997) Plant regeneration from protoplasts of Capsicum annuum L cv. California Wonder. In: Journal of bioscience, 22 (3). pp. 339-344.

[img]
Preview
PDF
2.pdf - Published Version

Download (441kB)
Official URL: http://www.ias.ac.in/jbiosci/back.html

Abstract

Plant regeneration from mesophyll protoplasts of pepper, Capsicum annuum L. cv. California Wonder has been demonstrated via shoot organogenesis, Protoplasts isolated from fully expanded leaves of 3-week-old axenic shoots when cultured in TM medium supplemented with 1 mgl(-1) NAA, 1 mgl(-1) 2, 4-D, 0.5 mgl(-1) BAP (CM 1) resulted in divisions with a frequency ranging from 20-25%. Antioxidant ascorbic acid and polyvinylpyrrolidone (PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed in TM medium containing 2 mgl(-1) NAA and 0.5 mgl(-1) BAP (CM II) and MS gelled medium containing 2 mgl(-1) NAA and 0.5 mgl(-1) BAP (CM III), respectively, Regeneration of plantlets was possible via caulogenesis, Microshoots, 2-5 per callus appeared on MS gelled medium enriched with 0.5 mgl(-1) IAA, 2 mgl(-1) GA and 10 mgl(-1) BAP (CM IVc). Rooting of microshoots was obtained on half strength gelled medium containing 1 mgl(-1) NAA and 0.5 mgl(-1) BAP, Protoplasts isolated from cotyledons failed to divide and degenerated eventually.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to Indian academy of sciences.
Keywords: leaf protoplasts;Capsicum annuum L cv California Wonder; regeneration;organogenesis.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Mr. Khaleel Ahmed Haveri
Date Deposited: 27 Jan 2010 08:20
Last Modified: 19 Sep 2010 05:51
URI: http://eprints.iisc.ac.in/id/eprint/24689

Actions (login required)

View Item View Item