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Immunological studies on nucleic-acids and their components .6. Antibodies specific to two deoxyribotrinucleotide sequences

Khan, Saleem A and Jacob, TM (1977) Immunological studies on nucleic-acids and their components .6. Antibodies specific to two deoxyribotrinucleotide sequences. In: Nucleic Acids Research, 4 (9). pp. 3007-3016.

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Official URL: http://nar.oxfordjournals.org/cgi/content/abstract...

Abstract

Antibodies to the deoxyribotrinucleotides dpApTpA and dpApApT were prepared by injecting the bovine serum albumin conjugates of the respective haptens in rabbits. The specificities of the antibodies were determined by estimating the inhibition of the binding of the tritiated haptens to the immunoglobulins by various nonradioactive mono- and oligonucleotides, using nitrocellulose membrane binding assay. Anti-dpApTpA and anti-dpApApT antisera were found to contain antibodies which were highly specific to the respective hapten sequence.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Oxford University Press.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Paramesha S
Date Deposited: 21 Jan 2010 06:57
Last Modified: 21 Jan 2010 06:57
URI: http://eprints.iisc.ac.in/id/eprint/24391

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