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Sequence of reactions leading to cytochrome-p-450 synthesis-effect of drugs

Rajamanickam, Chellam and Rao, Manchanahalli R Satyanarayana and Padmanaban, Govindarajan (1975) Sequence of reactions leading to cytochrome-p-450 synthesis-effect of drugs. In: Journal of Biological Chemistry, 250 (6). pp. 2305-2310.

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Official URL: http://www.jbc.org/content/250/6/2305.abstract

Abstract

The effect of phenobarbital on the rates of the synthesis of the protein and heme moieties of cytochrome P-450 has been studied. For this purpose, cytochrome P-450 has been partially purified as its P-420 derivative and the labeled amino acid incorporation into the protein has been studied after subjecting a partially purified preparation to sodium dodecyl sulfate gel electrophoresis. The incorporation studies into the protein species after sodium dodecyl sulfate gel electrophoresis reveal that the drug primarily accelerates the rate of apoprotein synthesis followed by an increase in the rate of heme synthesis. The messenger for apocytochrome P-450 appears to be fairly stable.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: K.S. Satyashree
Date Deposited: 22 Jan 2010 05:07
Last Modified: 19 Sep 2010 05:49
URI: http://eprints.iisc.ac.in/id/eprint/24263

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