Layer-by-layer self-assembly of modified hyaluronic acid/chitosan based on hydrogen bonding

Manna, Uttam and Bharani, Sri and Patil, Satish (2009) Layer-by-layer self-assembly of modified hyaluronic acid/chitosan based on hydrogen bonding. In: Biomacromolecules, 10 (9). pp. 2632-2639.

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Abstract

The fabrication of hydrogen bonded polymer self-assembly for drug delivery has been accomplished via layer-by-layer sequential assembly from aqueous solution. In this study, the self-assembly was constructed based on hydrogen bonding between DNA base (adenine and thymine) pairs substituted on the backbone of chitosan and hyaluronic acid. Chitosan was modified with adenine, whereas hyaluronic acid was modified with thymine. Subsequently, these two polymers were sequentially absorbed on flat substrate by taking advantage of interactions of DNA base pairs via hydrogen bonding. Interlayer hydrogen bonding of these two polymers produces stable multilayer film without using any cross-linking agent. Thin film formation on quartz substrate has been monitored with UV-vis spectra and an AFM study. Formation of multilayer hydrogen-bonded thin film has been further confirmed with SEM. Encapsulation and release behavior of the therapeutic drug from the multilayer thin film at different conditions has been illustrated using UV-vis spectra. Cell viability of modified polymers using MTT assay confirmed no cytotoxic effect.

Item Type:	Journal Article
Publication:	Biomacromolecules
Publisher:	American Chemical Society
Additional Information:	Copyright of this article belongs to American Chemical Society.
Keywords:	Dna multilayer films;chitosan derivatives;release properties; drug-delivery;chitin;ph;nanoparticles;microencapsulation; biodegradability;microcapsules.
Department/Centre:	Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited:	16 Dec 2009 07:01
Last Modified:	19 Sep 2010 05:46
URI:	http://eprints.iisc.ac.in/id/eprint/23485

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