Studies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo

Anandaraj, MP Jacob Spurgeon and Cherayil, Joseph D (1974) Studies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo. In: Biochemical Journal, 141 (3). pp. 647-653.

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Abstract

Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T1 ribonuclease digest of this purified tRNA gave a simple chromatographic pattern. Because of the simplicity of the method of preparation of tRNA from this readily available source and the presence of only a few species of tRNA for each amino acid, chick embryo is suited for the study of tRNA and its various functions in higher systems.

Item Type:	Journal Article
Publication:	Biochemical Journal
Publisher:	Portland Press
Additional Information:	Copyright of this article belongs to Portland Press.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	24 Dec 2009 09:04
Last Modified:	19 Sep 2010 05:44
URI:	http://eprints.iisc.ac.in/id/eprint/23148

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