Evidence for Non-coordinated synthesis of 5 S RNA in the thermophilic fungus, Thermomyces lanuginosus

Rao, S Nageswara and Cherayil, Joseph D (1982) Evidence for Non-coordinated synthesis of 5 S RNA in the thermophilic fungus, Thermomyces lanuginosus. In: Journal of Biosciences, 4 (2). pp. 239-244.

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Abstract

Analysis of ribosomes and the post ribosomal supernatant fraction of actively growing cells ofThermomyces lanuginosus showed the presence of free 5 S RNA in the supernatant fraction. This 5 S RNA was identical to the ribosomal 5 S RNA in its electrophoretic mobility on 10% Polyacrylamide gel and in its base composition. 5 S RNA from both the sources gave evidence for the presence of diphosphate at the 5’ end. Most of the 5 S RNA that appeared in the cytoplasm was that transported from the nucleus during the isolation. This could be prevented by the use of a hexylene glycol-HEPES buffer.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Springer India, in co-publication with Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	5 S RNA - thermophilic fungus - ribosomal RNA - non-coordinated synthesis of PNA
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	24 Dec 2009 11:13
Last Modified:	19 Sep 2010 05:43
URI:	http://eprints.iisc.ac.in/id/eprint/22896

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