Biradicals as ESR Probes of Conformations in Model \beta-turn Peptides

Sankarapandi, S and Sukumar, M and Balaram, P and Manoharan, PT (1995) Biradicals as ESR Probes of Conformations in Model \beta-turn Peptides. In: Biochemical and Biophysical Research Communications, 213 (2). pp. 439-446.

PDF biradicals.pdf Restricted to Registered users only Download (327kB) | Request a copy

Abstract

Electron spin-spin exchange interaction in biradicals has been examined for its potential usefulness in the conformational analysis of peptides in solution. Three peptides with high propensity to adopt \beta-turn conformations in solution, Boc-Cys-Pro-Xxx-Cys-NHMe (Xxx = Leu, 1; Xxx = Aib, 2; Xxx = Tyr, 3), have been modified into biradicals by spin labeling the thiols groups. Analysis of electron spin resonance spectra of these peptides in it variety of solvents and at different temperatures suggests that the population of folded conformations follows the order 2 > 1 > 3.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright for this article belongs to Elsevier.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	08 Nov 2004
Last Modified:	19 Sep 2010 04:17
URI:	http://eprints.iisc.ac.in/id/eprint/2274

Actions (login required)

View Item