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Effect of glycosylation on the structure of Erythrina corallodendron lectin

Kulkarni, KA and Srivastava, A and Mitra, N and Sharon, N and Surolia, A and Vijayan, M and Suguna, K (2004) Effect of glycosylation on the structure of Erythrina corallodendron lectin. In: Proteins-Structure Function and Bioinformatics, 56 (4). pp. 821-827.

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/prot.20...

Abstract

The three-dimensional structure of the recombinant form of Erythrina corallodendron lectin, complexed with lactose, has been elucidated by X-ray crystallography at 2.55 Å resolution. Comparison of this non-glycosylated structure with that of the native glycosylated lectin reveals that the tertiary and quaternary structures are identical in the two forms, with local changes observed at one of the glycosylation sites (Asn17). These changes take place in such a way that hydrogen bonds with the neighboring protein molecules in rECorL compensate those made by the glycan with the protein in ECorL. Contrary to an earlier report, this study demonstrates that the glycan attached to the lectin does not influence the oligomeric state of the lectin. Identical interactions between the lectin and the non-covalently bound lactose in the two forms indicate, in line with earlier reports, that glycosylation does not affect the carbohydrate specificity of the lectin. The present study, the first of its kind involving a glycosylated protein with a well-defined glycan and the corresponding deglycosylated form, provides insights into the structural aspects of protein glycosylation.

Item Type: Journal Article
Publication: Proteins-Structure Function and Bioinformatics
Publisher: John Wiley and Sons
Additional Information: The copyright of this article belongs to John Wiley and Sons.
Keywords: legume lectin;glycosylation;crystal structure;Erythrina lectin;quaternary structure
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 03 Dec 2004
Last Modified: 17 Jan 2012 07:15
URI: http://eprints.iisc.ac.in/id/eprint/2223

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