Regulation of cytochrome P-450 gene expression. Studies with a cloned probe

Ravishankar, H and Padmanaban, G (1985) Regulation of cytochrome P-450 gene expression. Studies with a cloned probe. In: Journal of Biological Chemistry, 260 (3). pp. 1588-1592.

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Abstract

A cDNA clone for cytochrome P-450e, a phenobarbitone-inducible species in rat liver, has been isolated and characterized. With the use of this cloned DNA, an attempt has been initiated to elucidate the factors regulating the cytochrome P-450 gene expression. Inhibitors of heme synthesis such as cobalt chloride and 3-amino-1,2,4-triazole block the induction of cytochrome P-450e by phenobarbitone at the level of transcription. This is evident from the decrease in the rate of synthesis of cytochrome P-450e, a decrease in the levels of specific translatable messenger RNA, a decrease in the specific cytoplasmic and nuclear messenger RNA contents, and nuclear transcription of cytochrome P-450e gene, as revealed by hybridization to the cloned probe, under these conditions. It is proposed that heme is a general regulator of cytochrome P-450 gene expression at the level of transcription, whereas the drug or its metabolite would impart the specificity needed for the induction of a particular species.

Item Type:	Journal Article
Publication:	Journal of Biological Chemistry
Publisher:	Amer Soc Biochemistry Molecular Biology Inc
Additional Information:	Copyright of this article belongs to Amer Soc Biochemistry Molecular Biology Inc.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	27 Jan 2010 10:22
Last Modified:	19 Sep 2010 05:41
URI:	http://eprints.iisc.ac.in/id/eprint/22223

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