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Modulation of arginine decarboxylase activity in cucumber (Cucumis sativus) cotyledons in short-term organ culture

Prasad, GL and Adiga, PR (1985) Modulation of arginine decarboxylase activity in cucumber (Cucumis sativus) cotyledons in short-term organ culture. In: Journal of Plant Growth Regulation, 4 (1-4). pp. 49-61.

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Abstract

Among the various amines administered to excisedCucumis sativus cotyledons in short-term organ culture, agmatine (AGM) inhibited arginine decarboxylase (ADC) activity to around 50%, and putrescine was the most potent entity in this regard. Homoarginine (HARG) dramatically stimulated (3- to 4-fold) the enzyme activity. Both AGM inhibition and HARG stimulation of ADC were transient, the maximum response being elicited at 12 h of culture. Mixing experiments ruled out involvement of a macromolecular effector in the observed modulation of ADC. HARG-stimulated ADC activity was completely abolished by cycloheximide, whereas AGM-mediated inhibition was unaffected. Half-life of the enzyme did not alter on treatment with either HARG or AGM. The observed alterations in ADC activity are accompanied by change in Km of the enzyme. HARG-stimulated ADC activity is additive to that induced by benzyladenine (BA) whereas in presence of KCl, HARG failed to enhance ADC activity, thus demonstrating the overriding influence of K+ on amine metabolism.

Item Type: Journal Article
Additional Information: The copyright of this article belongs to Springer.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Paramesha S
Date Deposited: 27 Nov 2009 09:04
Last Modified: 01 Mar 2012 06:20
URI: http://eprints.iisc.ac.in/id/eprint/22034

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