Microsomal 11-Alpha-hydroxylation of progesterone in Aspergillius ochraceus: Part I: Characterization of the hydroxylase system

Jayanthi, CR and Madyastha, Prema and Madyastha, K Madhava (1982) Microsomal 11-Alpha-hydroxylation of progesterone in Aspergillius ochraceus: Part I: Characterization of the hydroxylase system. In: Biochemical and Biophysical Research Communications, 106 (4). pp. 1262-1268.

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Abstract

Microsomes (105,000xg sediment) prepared from induced cells of A.ochraceus was found to hydroxylate progesterone to 11-Alpha-hydroxyprogesterone (11a-OHP) in high yields (85-�90% in 30 min.) in the presence of NADPH and O2. The pH optimum for the hydroxylase was found to be 7.7. However, for the isolation of active microsomes grinding of the mycelium should be carried out at pH 8.3. Metyrapone, carbon monoxide, SKF-525A, p-CMB and N-methyl maleimide inhibited the hydroxylase activity indicating the involvement of cytochrome P-450 system. The inhibition of the hydroxylase by cytochrome Image and the presence of high levels of NADPH-cytochrome Image reductase in induced microsomes suggest that the reductase could be one of the components in the hydroxylase system.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	29 Dec 2009 11:39
Last Modified:	19 Sep 2010 05:37
URI:	http://eprints.iisc.ac.in/id/eprint/21455

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