Interaction of Cibacron Blue F3G-A and Procion Red HE-3B with sheep liver 5,10-methylenetetrahydrofolate reductase

Varalakshmi, Satyam K and Savithri, ΗS and Rao, N Appaji (1987) Interaction of Cibacron Blue F3G-A and Procion Red HE-3B with sheep liver 5,10-methylenetetrahydrofolate reductase. In: Journal of Biosciences, 11 (1-4). pp. 265-274.

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Abstract

Cibacron Blue F3G-A, a probe used to monitor nucleotide binding domains in enzymes, inhibited sheep liver 5, 10-methylenetetrahydrofolate reductase competitively with respect to 5-methyltetrahydrofolate and NADPH. The Ki values obtained by kinetic methods and the Kd value for the binding of the dye to the enzyme estimated by protein fluorescence quenching were in the range 0·9-1·2 μM. Another triazine dye, Procion Red HE-3B interacted with the enzyme in an essentially similar manner to that observed with Cibacron Blue F3G-A. These results as well as the interaction of the dye with the enzyme monitored by difference spectroscopy and intrinsic protein fluorescence quenching methods indicated that the dye was probably interacting at the active site of the enzyme by binding at a hydrophobic region.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright of this article belongs to Indian Academy of Sciences.
Keywords:	Cibacron Blue F3G-A;Procion Red HE-3B;5,10-methylenetetrahydrofolate reductase.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	25 Aug 2009 12:10
Last Modified:	19 Sep 2010 05:36
URI:	http://eprints.iisc.ac.in/id/eprint/21309

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