Polymorphism in B-DNA - X-ray-Diffraction Studies on Li-DNA Fibers

Parrack, P and Sundaramoorthy, M and Sasisekharan, V (1985) Polymorphism in B-DNA - X-ray-Diffraction Studies on Li-DNA Fibers. In: Journal of Bioscience, 8 (3-4). pp. 507-516.

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Abstract

From X-ray diffraction studies it is generally believed that B-DNA has the structural parameters n = 10 and h = 3.4 Å. However, for the first time we report that polymorphism in the B-form can be observed in DNA fibres. This was achieved by the precise control of salt and humidity in fibres and by the application of the precession method of X-ray diffraction to DNA fibres. The significant result obtained is that n = 10 is not observed for crystalline fibre patterns. In fact, n = 10 and h = 3.4 Å are not found to occur simultaneously. Instead, a range of values, n = 9.6–10.0 and h = 3.35 Å–3.41 Å is observed.

Item Type:	Journal Article
Publication:	Journal of Bioscience
Publisher:	Indian Academy Science
Additional Information:	Copyright of this article belongs to Indian Academy Science
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	06 Nov 2009 09:01
Last Modified:	19 Sep 2010 05:31
URI:	http://eprints.iisc.ac.in/id/eprint/20060

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