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Cloning and development of pathotype-specific SCAR marker associated with Sclerospora graminicola isolates from pearl millet

Sudisha, J and Kumar, S Ananda and Niranjana, SR and Shetty, NP and Shetty, HShekar (2009) Cloning and development of pathotype-specific SCAR marker associated with Sclerospora graminicola isolates from pearl millet. In: Australasian Plant Pathology, 38 (3). pp. 216-221.

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Official URL: http://www.publish.csiro.au/nid/39/paper/AP08098.h...

Abstract

Downy mildew pathogen of pearl millet in India is associated with the spread of the highly virulent Sclerospora graminicola pathotype-1. Twenty-seven S. graminicola isolates were screened using 20 intersimple sequence repeats (ISSR). Dinucleotide repeat primer [17898A-(CA)(6) AC] amplified a similar to 600 bp fragment specific to five isolates of pathotype-1 (Sg 048, Sg 153, Sg 212, DM-11 and DM-90). The ISSR fragment linked with pathotype-1 was cloned successfully and sequenced. To convert ISSR fragments into pathotype-specific sequence characterised amplified region (SCAR) markers, PCR primers were designed using a sequence of the cloned DNA fragment. PCR amplification using SCAR primer pair (UOM3-Sg-Path1-F/R) amplified a single 284 bp band only in isolates of S. graminicola pathotype-1. This SCAR primer pair did not amplify the 284 bp product from the other five S. graminicola pathotypes or a negative control, which demonstrates primer specificity for pathotype-1. The SCAR primer pair (UOM3-Sg-Path1-F/R) obtained in this study will provide a valuable tool for rapid identification and specific detection of S. graminicola pathotype-1.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Commonwealth Scientific and Industrial Research Organisation Publishing.
Keywords: PCR;Pennisetum glaucum;SCAR markers.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Id for Latest eprints
Date Deposited: 28 May 2009 10:05
Last Modified: 28 May 2009 10:05
URI: http://eprints.iisc.ac.in/id/eprint/19847

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