ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Conformational Changes Triggered by Mg2+ Mediate Transactivator Function

Swapna, Ganduri and Saravanan, Matheswaran and Nagaraja, Valakunja (2009) Conformational Changes Triggered by Mg2+ Mediate Transactivator Function. In: Biochemistry, 48 (11). pp. 2347-2354.

[img] PDF
fulltext.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy
Official URL: http://pubs.acs.org/doi/pdf/10.1021/bi8022448


Transactivator protein C of bacteriophage mu is essential for the transition from middle to late gene expression during the phage life cycle. The unusual, multistep activation of mom promoter (Pmom) by C protein involves activator-mediated promoter unwinding to recruit RNA polymerase and subsequent enhanced promoter clearance of the enzyme. To achieve this, C binds its site overlapping the -35 region of the mom promoter with a very high affinity, in Mg2+-dependent fashion. Mg2+-mediated conformational transition in C is necessary for its DNA binding and transactivation. We have determined the residues in C which coordinate Mg2+, to induce allosteric transition in the protein, required for the specific interaction with DNA. Residues E26 and D40 in the putative metal binding motif (E26X10D37X2D40) present toward the N-terminus of the protein are found to be important for Mg2+ ion binding. Mutations in these residues lead to altered Mg2+-induced conformation, compromised DNA binding, and reduced levels of transcription activation. Although Mg2+ is widely used in various DNA transaction reactions, this report provides the first insights on the importance of the metal ion-induced allosteric transitions in regulating transcription factor function.

Item Type: Journal Article
Publication: Biochemistry
Publisher: American Chemical Society
Additional Information: Copyright of this article belongs to American Chemical Society.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 01 Jun 2009 10:26
Last Modified: 19 Sep 2010 05:30
URI: http://eprints.iisc.ac.in/id/eprint/19751

Actions (login required)

View Item View Item