Estimation of mineral-adhered biomass of Thiobacillusferrooxidans by protein assay-some problems and remedies

Karan, Goutam and Natarajan, KA and Modak, JM (1996) Estimation of mineral-adhered biomass of Thiobacillusferrooxidans by protein assay-some problems and remedies. In: Hydrometallurgy, 42 (02). pp. 169-175.

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Abstract

Enumeration of adhered cells of Thiobacillus ferrooxidans on sulphide minerals through protein assay poses problems due to interference from dissolved mineral constituents. The manner in which sulphide minerals such as pyrite, chalcopyrite, sphalerite, arsenopyrite and pyrrhotite interfere with bacterial protein estimation is demonstrated. Such interferences can be minimised either through dilution or addition of H2O2 to the filtrate after hot alkaline digestion of the biotreated mineral samples.

Item Type:	Journal Article
Publication:	Hydrometallurgy
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Mechanical Sciences > Chemical Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	17 Jul 2009 09:21
Last Modified:	19 Sep 2010 05:29
URI:	http://eprints.iisc.ac.in/id/eprint/19707

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