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Identification of actin as an estradiol 17-beta-stimulated protein in the human placenta

Sudha, S and Kumari, U and Rao, VS and Rao, AJ (1997) Identification of actin as an estradiol 17-beta-stimulated protein in the human placenta. In: Biochemistry And Molecular Biology International, 43 (5). pp. 955-966.

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Official URL: http://www.informaworld.com/smpp/content~db=all~co...

Abstract

Addition of estradiol 17-beta to first trimester human placental minces resulted in an increased synthesis of a protein of apparent molecular weight 45 kDa. The specific involvement of estrogen in the stimulation of this protein was established by demonstrating a reduction in the level of this protein by the addition of CCS 16949 A, an inhibitor of aromatase, a key enzyme in the biosynthesis of estradiol 17-beta and ICI 182,780, an estrogen receptor antagonist. The protein was purified to homogeneity and N-terminal sequencing of two of the internal peptides obtained by enzymatic digestion of the protein, as well as the absence of a free N-terminal indicated that it could be actin. This was confirmed by Western blotting using commercially available actin antiserum. The role of estradiol 17-beta in the stimulation of actin synthesis in human placenta was also established by monitoring the quantitative inhibition of DNase I by actin.

Item Type: Journal Article
Additional Information: Copyright of this article belongs Taylor and Francis Group.
Keywords: Actin;cytoskeleton;DNase I;estradiol 17-beta;placenta;pregnancy.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Mr. S Muthuraja
Date Deposited: 17 Aug 2009 06:52
Last Modified: 17 Aug 2009 06:52
URI: http://eprints.iisc.ac.in/id/eprint/19378

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