Tetrapentylammonium block of chloramine-T and veratridine modified rat brain type iia sodium channels

Ghatpande, AS and Rao, S and Sikdar, SK (2001) Tetrapentylammonium block of chloramine-T and veratridine modified rat brain type iia sodium channels. In: British Journal of Pharmacology, 132 (8). pp. 1755-1760.

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Abstract

Tetrapentylammonium (TPeA) block of rat brain type IIA sodium channel alpha subunit was studied using whole cell patch clamp. Results indicate that TPeA blocks the inactivating brain sodium channel in a potential and use-dependent manner similar to that of the cardiac sodium channel. Removal of inactivation using chloramine-T (CT) unmasks a time-dependent block by TPeA consistent with slow blocking kinetics. On the other hand, no time dependence is observed when inactivation is abolished by modification with veratridine. TPeA does not bind in a potential-dependent fashion to veratridine-modified channels and does not significantly affect gating of veratridine-modified channels suggesting that high affinity binding of TPeA to the brain sodium channel is lost after veratridine modification

Item Type:	Journal Article
Publication:	British Journal of Pharmacology
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	15 Sep 2004
Last Modified:	13 Jan 2012 09:40
URI:	http://eprints.iisc.ac.in/id/eprint/1936

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