Spectroscopic studies of the interactions of the pyrethroid insecticide fenvalerate with gramicidin

Ghosh, JK and Sarkar, SN and Sikdar, SK (1998) Spectroscopic studies of the interactions of the pyrethroid insecticide fenvalerate with gramicidin. In: Biochemistry and Molecular Biology International, 44 (06). pp. 1083-1092.

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Abstract

Fenvalerate is a pyrethroid insecticide which interacts with ionic channels. Using circular dichroism technique we have studied the interaction of fenvalerate with gramicidin, a model channel peptide which transports ions. In most organic solvents, gramicidin exists as a double helix except in trifluoroethanol where it exists as a channel forming single stranded beta(6.3) helical monomer. In model lipid membranes, under certain experimental conditions, gramicidin exists as a channel forming single stranded beta(6.3) helical dimer. Our results show that fenvalerate interacts more with the single stranded beta(6.3) helical monomer or dimer than with the double helical form of gramicidin. This was further confirmed by an increase in the rate of gramicidin mediated proton transport in liposomes by fenvalerate, using the pH sensitive fluorophore, pyranine.

Item Type:	Journal Article
Publication:	Biochemistry and Molecular Biology International
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Keywords:	fenvalerate;gramicidin;liposome;circular dichroism;pyranine; proton flux.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	21 Dec 2009 09:19
Last Modified:	19 Sep 2010 05:27
URI:	http://eprints.iisc.ac.in/id/eprint/19264

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