Determination of kinetic parameters of epitope-paratope interaction based on solid phase binding: An inexpensive alternate to biospecific interaction analysis

Murthy, GS and Venkatesh, N (1996) Determination of kinetic parameters of epitope-paratope interaction based on solid phase binding: An inexpensive alternate to biospecific interaction analysis. In: Journal of Bioscience, 21 (05). pp. 641-651.

PDF Determination.pdf - Published Version Restricted to Registered users only Download (287kB) | Request a copy

Abstract

A method has been developed for biospecific interaction analysis between antigen and antibody using solid phase binding approach. Real time kinetics between monoclonal antibody and human chorionic gonadotropin have been studied. Kinetic constants of the bimolecular reaction are determined Affinity constants measured by several independent methods have been found to be relatively consistent Convenient and simple procedures to determine affinity constant, K-on and K-off of monoclonal antibody-human chorionic gonadotropin interaction using binding of [I-125]hCG to immobilized monoclonal antibody are presented. Values obtained compare well with these obtained using surface plasmon resonance technology, making this method a viable alternative.

Item Type:	Journal Article
Publication:	Journal of Bioscience
Publisher:	Indian Academy Sciences
Additional Information:	Copyright of this article belongs to Indian Academy Sciences.
Keywords:	Antigen-antibody interaction;association rate constants;dissociation rate constants;affinity constant; biospecific interaction analysis;human chorionic gonadotropin
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	30 May 2009 17:51
Last Modified:	19 Sep 2010 05:26
URI:	http://eprints.iisc.ac.in/id/eprint/18985

Actions (login required)

View Item