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Mycobacterium tuberculosis RuvA Induces Two Distinct Types of Structural Distortions between the Homologous and Heterologous Holliday Junctions

Khanduja, Jasbeer Singh and Tripathi, Pankaj and Muniyappa, K (2009) Mycobacterium tuberculosis RuvA Induces Two Distinct Types of Structural Distortions between the Homologous and Heterologous Holliday Junctions. In: Biochemistry, 48 (01). pp. 27-40.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/bi8016526

Abstract

A central step in the process of homologous genetic recombination is the strand exchange between two homologous DNA molecules, leading to the formation of the Holliday junction intermediate. Several lines of evidence, both in vitro and in vivo, suggest a concerted role for the Escherichia coli RuvABC protein complex in the process of branch migration and the resolution of the Holliday junctions. A number of investigations have examined the role of RuvA protein in branch migration of the Holliday junction in conjunction with its natural cellular partner, RuvB. However, it remains unclear whether the RuvABC protein complex or its individual subunits function differently in the context of DNA repair and homologous recombination. In this study, we have specifically investigated the function of RuvA protein using Holliday junctions containing either homologous or heterologous arms. Our data show that Mycobocterium tuberculosis ruvA complements E. coli Delta ruvA mutants for survival to genotoxic stress caused by different DNA-damaging agents, and the purified RuvA protein binds HJ in preference to any other substrates. Strikingly, our analysis revealed two distinct types of structural distortions caused by M. tuberculosis RuvA between the homologous and heterologous Holliday junctions. We interpret these data as evidence that local distortion of base pairing in the arms of homologous Holliday junctions by RuvA might augment branch migration catalyzed by RuvB. The biological significance of two modes of structural distortion caused by M. tuberculosis RuvA and the implications for its role in DNA repair and homologous recombination are discussed.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to American Chemical Society .
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Arun
Date Deposited: 07 Jan 2010 08:56
Last Modified: 19 Sep 2010 05:25
URI: http://eprints.iisc.ac.in/id/eprint/18771

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