Identification and mapping of linear antigenic determinants of chicken riboflavin carrier protein

Subramanian, Sarada and Adiga, Radhakantha P (1998) Identification and mapping of linear antigenic determinants of chicken riboflavin carrier protein. In: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1429 (01). pp. 74-82.

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Abstract

All of the possible 212 overlapping octapeptides covering the amino acid sequence of chicken egg white riboflavin carrier protein (RCP) was synthesized on polyethylene rods. To identify the peptides constituting the sequential antigenic epitopes on chicken RCP, a PEPSCAN ELISA was employed where antibodies raised against SDS-treated, reduced and carboxymethylated RCP (RCM-RCP) in mice, rats (n = 4), rabbits and monkeys (n = 3) were used. By this approach, we could identify five sequences on chicken RCP commonly recognized by all the antisera from these species which were spanning the residues 10-17, 42-4, 134-141, 172-179 and 200-207. Analysis of the data for each individual antiserum indicated inter- and intra-species variations with N- and C-terminal shifts in peak reactivity. The present study offers clues for development of potential immunocontraceptive vaccines.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	carboxymethylated riboflavin carrier protein;polyclonal antibody;peptide scanning;common epitope.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	12 Aug 2009 10:58
Last Modified:	01 Mar 2012 07:01
URI:	http://eprints.iisc.ac.in/id/eprint/18720

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