Recombinant expression of human transforming growth factor-beta isoforms in Chinese hamster ovary cells

Ramani, B and Kondaiah, P (1998) Recombinant expression of human transforming growth factor-beta isoforms in Chinese hamster ovary cells. In: Journal of Biosciences, 23 (5). pp. 577-583.

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Abstract

Transforming growth factor-beta s (TGF-beta s) are multi functional growth modulators implicated in several physiological processes which include embryogenesis, inflammation, immune-suppression, wound healing, carcinogenesis and cellular differentiation. For clinical use, recombinant expression of TGF-beta s is the only practical source because of very low yields from natural sources. Here, we report the recombinant expression of human TGF-beta 1 and TGF-beta 2 in a mammalian expression system using a high expression eukaryotic vector driven by a cytomegalovirus promoter. Expression levels are as high as 0.97 mu g/ml of TGF-beta 1 and 0.24 mu g/ml of TGF-beta 2 in conditioned media, sufficient for purification without the need for amplification of the gene using methotrexate.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy Sciences
Additional Information:	Copyright for this article belongs to Indian Academy of Sciences.
Keywords:	Recombinant DNA;gene expression;growth factors;TGF-beta;CHO cells
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	04 Oct 2004
Last Modified:	19 Sep 2010 04:15
URI:	http://eprints.iisc.ac.in/id/eprint/1641

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