Immunobiology of a Synthetic Luteinizing Hormone Receptor Peptide 21–41

Moudgal, NR and Krishnamurthy, HN and Surekha, S and Krishnamurthy, H and Dhople, VM and Nagaraj, R and Sairam, MR (2001) Immunobiology of a Synthetic Luteinizing Hormone Receptor Peptide 21–41. In: Journal of Andrology, 22 (6). pp. 992-998.

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Abstract

Immunization of adult male rabbits with a synthetic luteinizing hormone-receptor peptide (LH-RP; representing amino-acids 21-41 of the extracellular domain of the rat LH receptor) resulted in production of high-titer antibodies capable of interacting with particulate and cell-based LH receptors. The antibody produced was able to inhibit binding of I-125-labeled human chorionic gonadotropin (hCG) to a particulate sheep luteal LH receptor preparation by 40%-50%. Maximal inhibitory activity was correlated with high antibody titer. Immunocytometry revealed that the antibody could directly bind to cells having LH receptors, such as rat granulosa and Leydig cells. The antibodies recognized a 77-kilodalton membrane protein in Western blots of mouse testicular extracts. Interaction of endogenous Leydig cell LH receptor with the LH-RP antibody resulted in both hormone agonist and antagonistic activities. The hormone-mimicking activity (increase in serum testosterone over control) was confined only to the early phase of immunization when the antibody titer was low. Blockade of LH receptor during the later part of immunization resulted in a significant reduction in serum testosterone over controls and inhibition of spermatogenesis. DNA flow cytometry showed that a specific and significant inhibition of meiosis (transformation of primary spermatocytes to round and elongated spermatids P < .01) and spermiogenesis (transformation of round spermatids to elongated permatids P < .0001) occurred following blockade of LH function.

Item Type:	Journal Article
Publication:	Journal of Andrology
Publisher:	American Society of Andrology
Additional Information:	Copyright of this article belongs to American Society of Andrology.
Keywords:	Testicular function;spermatogenesis;receptor antibodies.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	14 Oct 2008 10:02
Last Modified:	19 Sep 2010 04:50
URI:	http://eprints.iisc.ac.in/id/eprint/16057

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