Intramolecular i-motif Structure at Acidic pH for Progressive Myoclonus Epilepsy (EPM1) Repeat $d(CCCCGCCCCGCG)_n$

Pataskar, Shashank S and Dash, Debasis and Brahmachari, Samir K (2001) Intramolecular i-motif Structure at Acidic pH for Progressive Myoclonus Epilepsy (EPM1) Repeat $d(CCCCGCCCCGCG)_n$. In: Journal of Biomolecular Structure & Dynamics,, 19 (2). pp. 307-314.

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Abstract

The most common mutation associated with Progressive Myoclonus Epilepsy (EPM1) of Unverricht-Lundberg type is the expansion of a dodecamer repeat, d(CCCCGCCCCGCG)n. We show that the C-rich strand of this repeat (2-3 copies) forms intercalated i-motif structure at acidic pH as judged by CD spectroscopy and anomalous gel electrophoretic mobility. The stability of the structure increases with the increase in the length of the repeat. Transient formation of stable, folded back structure like i-motif could play an important role in the mechanism of expansion of this repeat.

Item Type:	Journal Article
Publication:	Journal of Biomolecular Structure & Dynamics,
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	17 Oct 2008 05:06
Last Modified:	19 Sep 2010 04:50
URI:	http://eprints.iisc.ac.in/id/eprint/16030

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