ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Recognition of B and Z forms of DNA by Escherichia coli DNA polymerase I

Ramesh, N and Shouche, Yogesh S and Brahmachari, Samir K (1986) Recognition of B and Z forms of DNA by Escherichia coli DNA polymerase I. In: Journal of Molecular Biology, 190 (4). pp. 635-638.

[img]
Preview
PDF
Recognition_of_B_and_Z_forms_of_DNA_by_Escherichia_coli_DNA_polymerase.pdf

Download (487kB)

Abstract

Since the substrate binding domain of the large proteolytic fragment of E.coli DNA polymerase I has been shown to interact with the B forms of DNA, the ability of this enzyme to recognize structures other than the B form was studied.The polymerase activity has been used to evaluate the degree of recognition of the B and Z forms of DNA.The Z form was found to promote less activity, indicating the probable inability of the polymerase to move along the conformationally rigid form of the template.The present study indicates that the Z-DNA found in vivo may have a role in the control of replication.

Item Type: Journal Article
Publication: Journal of Molecular Biology
Publisher: Elsevier Ltd.
Additional Information: Copyright belongs to Elsevier Ltd.
Keywords: DNA polymerase
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 09 May 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ac.in/id/eprint/13913

Actions (login required)

View Item View Item