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An avidin-biotin micro-enzymeimmunoassay for monkey chorionic gonadotrophin

Chakrabarti, R and Rao, Jagannadha A (1987) An avidin-biotin micro-enzymeimmunoassay for monkey chorionic gonadotrophin. In: Journal of Reproduction and Fertility, 80 (1). pp. 151-158.

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The avidin-biotin micro-enzymeimmunoassay used antiserum to the beta-subunit of sheep LH, biotinylated hCG as tracer and pooled serum from pregnant monkeys as a reference preparation of monkey CG. The assay was validated for specificity, by checking serum samples known to contain high quantities of LH, and false positive results were eliminated to a great extent by prior heating of serum samples at 60 degrees C for 30 min. In 45 bonnet monkeys the test gave 10 true positives, 34 true negatives and 1 false positive, i.e. an accuracy of 97%. The serum profiles of CG, measured by the EIA, in bonnet, cynomolgus and rhesus monkeys during early pregnancy were very similar to those reported for bonnet and rhesus monkeys when a liquid-phase radioimmunoassay was used.

Item Type: Journal Article
Publication: Journal of Reproduction and Fertility
Publisher: Society for Reproduction and Fertility
Additional Information: Copyright for this article belongs to the Society for Reproduction and Fertility.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 07 May 2008
Last Modified: 27 Aug 2008 13:21
URI: http://eprints.iisc.ac.in/id/eprint/13869

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