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Studies on the Interaction of Cupric Isonicotinohydrazide with DNA

Divakar, S and Vasudevachari, MB and Antony, A and Easwaran, KRK (1987) Studies on the Interaction of Cupric Isonicotinohydrazide with DNA. In: Biochemistry, 26 (13). pp. 3781-3785.

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The interaction of cupric isonicotinohydrazide $(Cu^{II}INH)$, an antiviral compound, with calf thymus DNA was investigated by circular dichroism (CD) and nuclear magnetic resonance (NMR). Gel electrophoresis of DNA incubated with $Cu^{II}INH$ showed cleavage of DNA to various extents. This cleavage was found to be time and concentration dependent. In the presence of $Cu^{II}INH$ the positive CD band at 274 nm disappeared and the negative band at 246 nm showed a decrease in the mean residual ellipticity value, indicating binding of $Cu^{II}INH$ to DNA. $^{31}P$ NMR studies indicated that the binding of copper in $Cu^{II}INH$ is to the phosphate oxygen of the DNA backbone. The binding of $Cu^{II}INH$ was also found to be reversible. Addition of ethylenediaminetetraacetic acid to the $Cu^{II}INH-DNA$ complex resulted in breaking of the complex and restoring the original structural features of the B family of DNA in the resulting fragments. At the concentration level of $Cu^{II}INH$ employed, both $CuS0_4$ and INH independently did not show any interaction with DNA.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to American Chemical Society.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Kiran Savanur
Date Deposited: 07 May 2008
Last Modified: 13 Feb 2019 09:09
URI: http://eprints.iisc.ac.in/id/eprint/13868

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