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Cloning and expression of the cDNA for the major antigen of foot and mouth disease virus type Asia I 63/72

Suryanarayana, VVS and Rao, BU and Padayatty, JD (1985) Cloning and expression of the cDNA for the major antigen of foot and mouth disease virus type Asia I 63/72. In: Current Science, 54 (20). pp. 1044-1048.

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Abstract

Double-stranded cDNA for the foot and mouth disease virus (FMDV) type Asia I63/72 was prepared and cloned in the expression vector pUR222. The recombinant DNA was transferred into Escherichia coli RRI-DT5 and the transformants were identified as white colonies in the presence of the dye x-gal on agar plates. Many of them gave strong pos. signals on hybridization with 32P-labeled viral RNA. The middle BamHI fragment of the cDNA is known to carry the sequence for a few nonstructural proteins and for the major antigen, VPI. The clones contg. the BamHI fragments of the cDNA and the nearly full length cDNA produced proteins which gave pos. signals in sandwich ELISA, showing that they cross-reacted with the antibodies raised against the virus. This showed that the major antigen is expressed by the cloned cDNA for the FMDV type Asia I.

Item Type: Journal Article
Additional Information: The copyright belongs to Indian Academy of Sciences
Keywords: Aphthovirus;Complementary DNA;Molecular cloning;Gene expression;Escherichia coli;Antigen;ELISA assay; Picornaviridae;Virus;Escherichieae;Enterobacteriaceae; Bacteria
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: RM Shivakumara
Date Deposited: 17 Apr 2008
Last Modified: 19 Sep 2010 04:44
URI: http://eprints.iisc.ac.in/id/eprint/13696

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