Identification and molecular characterization of a biotin-binding protein distinct from avidin of chicken egg white and comparison with yolk biotin-binding protein

Seshagiri, Polani B and Adiga, Radhakantha P (1987) Identification and molecular characterization of a biotin-binding protein distinct from avidin of chicken egg white and comparison with yolk biotin-binding protein. In: Biochimica et Biophysica Acta (BBA) - General Subjects, 926 (3). pp. 321-330.

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Abstract

By immunol. and biochem. methods a biotin-binding protein, distinct from avidin, was shown to be present in chicken egg white. This vitamin-binding protein (mol. wt. 67,000) bound [14C]biotin, displayed thermally induced biotin exchange reaction, and exhibited gross immunol. cross-reactivity with the purified yolk biotin-binding protein. In vitro labeling of sol. proteins with radioactive amino acids in the oviduct tissue explants from estrogenized chicks revealed that .apprx.2% of the total radioactive proteins immunopptd. with anti-yolk biotin-binding protein antibodies. The protein was purified to homogeneity by ion-exchange chromatog. on DEAE-cellulose and biotin-AH Sepharose affinity chromatog. The purified protein specifically bound [14C]biotin, and exhibited complete immunol. homol. with the yolk biotin-binding protein but not with avidin. Its electrophoretic mobility (at pH 8.3), acidic nature, biotin-binding characteristics, immunol. cross-reactivity, and tryptic peptide maps were very similar to those of yolk biotin-binding protein, and not avidin.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - General Subjects
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Biotin binding protein;Avidin (Chicken egg)
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	04 Mar 2008
Last Modified:	01 Mar 2012 07:31
URI:	http://eprints.iisc.ac.in/id/eprint/13155

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