Complex carbohydrate-lectin interaction at the interface: a model for cellular adhesion. I. Effect of vesicle size on the kinetics of aggregation between a fatty acid conjugate of lectin and a liposomal asialoganglioside

Chakrabarti, A and Podder, SK (1990) Complex carbohydrate-lectin interaction at the interface: a model for cellular adhesion. I. Effect of vesicle size on the kinetics of aggregation between a fatty acid conjugate of lectin and a liposomal asialoganglioside. In: Biochimica et Biophysica Acta (BBA) - Biomembranes, 1024 (1). pp. 103-110.

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Abstract

Two types of phospholipid vesicles capable of mutual recognition have been tailor-made to serve as a model system for the study of carbohydrate-mediated cellular adhesion. One of the vesicles contained a fatty acid conjugate of a galactose specific lectin (lectin vesicle_ and the other an asialoganglioside with a reactive terminal galactose residue (galactose vesicle). The kinetics of aggregation of these two types of vesicles was followed by monitoring time-dependent change in turbidity. A 10–100-fold enhancement in the forward rate constant ($k_f$ ranging from $7.1 · 10^5$ to$ 4.5 · 10^7 M^{-1} · s^{-1}$ at 27° C) was observed when compared with that for the lectin-galactose system in solution ($k_t$) being $4.5 · 10^5 M^-1 · s^-1)$, reported in the literature. A study of the influence of vesicle size on the rate of aggregation showed that enhancement depended on the curvature of the galactose vesicle rather than the density of asialoganglioside suggesting a possible diffusion in the plane of the membrane. The ratio,$ k_f/k_d$ is found to be approx. $10^{10} M^{-1}$ indicating that the formation of multiple bonds plays a role for stable adhesion.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher:	Elsevier Science Publishers B.V.
Additional Information:	copyright of this article belongs to Elsevier Science Publishers B.V.
Keywords:	Cellular adhesion;Liposome;Vesicle size;Asialoganglioside;Agglutinin
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	22 Feb 2008
Last Modified:	19 Sep 2010 04:41
URI:	http://eprints.iisc.ac.in/id/eprint/12715

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